Derivative/Conjugate
quantity
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abberior STAR 635, NHS ester, 1 mg


Item number
ST635-0002-1MG

Description

abberior STAR 635 is a dye developed for STED and confocal imaging in the red spectral region. It features exceptional photostability, very high brightness and low background labeling. abberior STAR 635 can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Our abberior STAR 635 can substitute dyes like ATTO® 647N, Alexa Fluor® 647, or Cy5®. Best results are obtained with freshly prepared samples.

The NHS ester is a widespread tool for conjugating a fluorescent dye to a protein or antibody. NHS ester can be used to label primary amines of proteins or amine-modified molecules. The resulting abberior conjugate will show a bright fluorescence and photostability. abberior offers protocols for labeling antibodies via amine-reactive groups.

Properties

Absorption
Absorption
635
Extinction Coefficient
Extinction Coefficient
110000
Emission
Emission
655
Quantum Efficiency
Quantum Efficiency
88
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
2.8
Correction Factor 260
Correction Factor 260
0.26
Correction Factor 280
Correction Factor 280
0.38
Charge
Charge
0
Molecular Weight
Molecular Weight
993.1
Derivative/Conjugate
NHS ester

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.